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Fig. 3
Depletion of leucocytes by PLX3397 treatment does not affect CM proliferation. (a,b) The impact of PLX3397 treatment on the presence of leucocytes was tested by labelling the hearts with L-plastin (red). (c,d) CM proliferation was quantified by measuring the number of CMs co-expressing cmlc:DsRed2-nuc (red) and MCM5 (green). Arrows indicate double-positive cells. (e) Quantification of L-plastin-positive area at 7 dpt. (f,g) Quantification of proliferative CM and non-CM nuclei at 7 dpt. (n ≥ 4 hearts; ≥2 sections per heart; ***p < 0.001; n.s. non-significant).
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