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Fig. 1
Expression of neogenin during zebrafish development. (a and b) neogenin mRNA expression was first detected by whole mount in situ hybridization at 14 hpf in the presumptive hindbrain (brackets) and in the lateral plate mesoderm (arrows). (c) At the onset of neurulation (16 hpf), neogenin expression was observed throughout the rostrocaudal extent of the neural rod (arrows) as well as in the lateral plate mesoderm (arrowheads). (d) At 18 hpf, neogenin expression localized to regions of the forebrain, midbrain, and hindbrain, including areas encompassing early clusters of differentiating neurons, for example, the dorsorostral cluster (drc) in the telencephalon and the ventrorostral cluster (vrc) in the rostral diencephalon. Expression was also detected in the rostral hindbrain (rhb) and tailbud (tb). (e) At 20 hpf, neogenin expression was found in the ventral portion of the most recently formed somites (arrows), tailbud, and the roof plate (rp) within the neural tube. (f-h) At 24 hpf, expression was observed in the lateral mesoderm (f, arrows) and (g) the neuroepithelium of the forebrain (fb), midbrain (mb), and hindbrain (hb). (h) neogenin expression was detected in neuronal clusters of the forebrain including in the telencephalon (t), diencephalon (d), including its dorsal region (dd), and the epiphysis (e). Meanwhile, midbrain expression was strong in the tegmentum (tg) but negligible in the tectum (te) and the midbrain component of the midbrain-hindbrain boundary (mhb). Strong expression was seen in the cerebellum (ce) and dorsal hindbrain. (a, b, d, e, and h) Lateral views with rostral to the left and dorsal to the top. (c, f, and g) Dorsal views with rostral to the left.
Reprinted from Developmental Biology, 269(1), Mawdsley, D.J., Cooper, H.M., Hogan, B.M., Cody, S.H., Lieschke, G.J., and Heath, J.K., The Netrin receptor Neogenin is required for neural tube formation and somitogenesis in zebrafish, 302-315, Copyright (2004) with permission from Elsevier. Full text @ Dev. Biol.