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Fig. 1
Structure and phylogenetic analysis of zebrafish PER2. (A) Alignment of zebrafish PER2 and PER3 amino acid sequences. Black boxes indicate identical residues and the underline denotes the PAS region. (B) Evolutionary conservation of phosphorylation sites in vertebrate PER2 proteins. The human Per2 sequence was corrected by introducing a nucleotide at position 3652 ( Toh et al., 2001). Open boxes denote putative phosphorylated Ser residues and the black box indicates the Ser662 residue, which is mutated in human FAPS syndrome. PER2 sequences used for the alignment were from zebrafish (AY171100), Xenopus (AF199499), quail (AB029890), human (AB002345). (C) Phylogenetic tree of the period protein family. Sequences used for the alignment were Drosophila PER (M30114), mouse PER1 (AB002108), mouse PER2 (AF035830), mouse PER3 (AF050182) zebrafish PER2 (AY171100) and zebrafish PER3 (AF254792) protein. Amino acid sequences were aligned using ClustalW. A phylogentetic tree of the Period gene family was derived using the neighbor-joining method with 1000 replicate searches. (D) RNase protection analysis of Per2 and EF1α mRNA expression in muscle of adult zebrafish. Yeast (Y) RNA was included as a negative control. Black and white bars indicate the dark and light phases, respectively (ZT, zeitgeber time).
Reprinted from Gene expression patterns : GEP, 3(3), Delaunay, F., Thisse, C., Thisse, B., and Laudet, V., Differential regulation of Period 2 and Period 3 expression during development of the zebrafish circadian clock, 319-324, Copyright (2003) with permission from Elsevier. Full text @ Gene Expr. Patterns