|
Fig. 8
Decreased habenular asymmetry is observed in Flh-deficient larvae. (A) Representative images of antibody labeling of krt4:eGFP (parapineal neurons) and Kctd12.1 protein (lateral habenular neurons) in the zebrafish epithalamus at 5dpf. Dorsal views. Scale bars: 30µm. (B,C) Asymmetry indices of Kctd12.1- (n=74; B) and Kctd12.2- (n=32; C) positive neurons in Flh-deficient larvae plotted against the numbers of parapineal neurons. The habenulae of larvae with the greatest reduction of Flh (flhn1-/-), and thus the largest number of parapineal neurons, were more symmetrical compared with their siblings. Pearson′s correlation tests were used to determine R2 values.