Fig. 2
tbx2b, flh and nr2e3 are expressed early during parapineal development. Dorsal views of in situ hybridizations of tbx2b, flh and nr2e3 in the pineal complex at the indicated stages. At 8 ss (13hpf), the pineal anlage is in the process of fusing at the midline. Parapineal specification occurs around 15-18hpf. By 24hpf, tbx2b-expressing parapineal precursors begin to coalesce at the anterior-most region of the pineal anlage. By 36hpf, a group of parapineal neurons can be observed as a distinct tbx2b-positive, flh/nr2e3-negative population that has migrated away from the pineal organ (arrowhead). Scale bar: 30µm.