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Fig. S5
Comparison of knockdown effects of different eph/ephrin genes on DFCs aggregation. (A-F) Representative DFCs patterns in morphants. Embryos were injected with individual morpholinos at the one-cell stage and fixed at the 75% epiboly stage for probing sox17 expression. Embryos were dorsally viewed. White arrows indicated presumably involuted DFCs-derived cells. MO doses: cMO, 10 ng; ephb3a-MO, 10 ng; ephb4b-MO2, 10 ng; efnb2a-MO, 10 ng; efnb2b-MO, 7 ng. (G)The ratios of embryos with different DFCs patterns (See Fig. 2N) were shown. n, the number of observed embryos.
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