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Fig. S5
shh induces rx3. (A,B) Wholemount views at 70% epiboly. rx3 is expressed in a broad crescent in anterior-most regions of the CNS. shh is expressed in ventral midline (vm) cells of the neural tube and in axial mesoderm. (B′) transverse section at the level of the prechordal plate (p.p), plane shown in (B). (C,D) Ventral views after rx3 wholemount in situ hybridization: rx3 expression is abolished in smu mutants (red arrowheads). (E,F) Number of tyroxine hydroxylase (th)-expressing cells in posterior hypothalamus is unchanged after cyclopamine treatment. Scale bars: 50µm.
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