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Fig. S2
Zones 1-III do not resolve in rx3-null embryos. Wholemount side or ventral views of 55hpf control (A.B) or morphant (C,D) embryos after in situ hybridization to detect rx3. Side and ventral views are aligned. Zones 1, II and III, readily distinguished in control embryos (white lines), cannot be resolved in morphant embryos. At the same time, the normal flexure of the hypothalamus is not detected in morphants (red dotted line and angle in (A) and (C)
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