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Fig. 6
Contact duration between DFCs and non-DFC cells is increased by deficiency of Eph/ephrin signaling. The membrane of embryonic cells from crosses of ephb4b+/-;Tg(sox17:GFP) fish was labeled by expressing exogenous CAAX-mCherry (red) and embryos were observed during midgastrulation. (A-D) Multi-focal and 3D reconstituted time-lapse images of different types of protrusions of DFCs in ephb4b mutant (Mt) (B) and WT siblings (A,C,D). The full process of a single protrusion is shown with time points indicated. The arrowheads indicate protrusions at the middle phase. See also Movies 8 and 9. (E-G) Round-trip time of protrusions. The start and end time points of the process for each protrusion were judged from single-focal, multi-focal and 3D movies. Only well-recognized protrusions were included. Np, number of protrusions; Ne, number of embryos.