ZFIN Image: Naylor et al., 2016, Fig. S7

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Figure Caption

Fig. S7

Distal tubule cells are positioned outside the non-convergence extension zone (NCEZ).

To test if distal tubule (DT) progenitors are positioned within the NCEZ, we uncaged embryos injected with a caged fluorescein dextran lineage label at the shield stage (6 hpf) in a position that encompassed the ventral-most 20° (immediately opposing the organizer). These embryos were left to develop to the 85% epiboly stage and then DT progenitors were labeled by uncaging at position (x³, y⁷). We find these progenitors are not positioned within the NCEZ. Top panels are dorsal views, bottom left panel is a lateral and bottom right panel is a ventral midline view. Scale bars represent 100µm

Acknowledgments

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