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Fig. 9 S1
Chemical inhibiton of PI3K, mTORC1 or NFkB rescues the epidermal malignancies, but not the pericardial edema of psoriasis mutants.
(a-h) Representative live images of psoriasis -/- embryos at 54 hpf, treated with the PI3K inhibitors Wortmannin (1 µM; c,c′), PIK90 (5 µM; d,d′) and LY 294002 (25 µM; e,e′), the mTORC1 inhibitors Rapamycin (1.1 µM; f,f′) and AZD8055 (30 µM; g,g′), and the NFκB inhibitor Withaferin A (30 µM; h, h′), as quantified in Figure 9e. Treatment with the different inhibitors of components of the PI3K-Akt-mTorC1-NFκB pathway do not rescue the pericardial edema (c- h, compare to wt (a) and non-treated mutant (b)), but do rescue the epidermal malignancies of mutant embryos (c′-h′, compare to wt (a′) and non-treated mutant (b′)). Scale bars: 500 µm (a), 250 µm (a′).