Fig. 5
Recovery in E3 medium after BTS immobilization induces structural changes in Candyfloss (Caf) larvae at 5 dpf.
Panel A-C shows birefringence microscopy of a Sibling (Sib), Sapje (Sap) and Caf larva from one-day E3 medium recovery. Panel D summarizes the corresponding force measurements of two mutant strains at 5dpf. Sap: filled bars; Caf: hatched bars; Sib: open bars. ***, p<0.001 compared to the Sib in DMSO, ##, p<0.01 compared to the Sap in DMSO, § §, p<0.01 compared to the Sib in BTS (ANOVA, Holm-Sidak method). No significant difference was detected between the BTS treated Sap groups. n = 3-10 in Sib groups; n = 3 in Sap and Caf groups.