ZFIN Image: Spicer et al., 2016, Fig. 8

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Figure Caption

Fig. 8

Adult gonadal morphology and gametogenesis do not differ between gnrh3^+/+ and gnrh3^-/- fish for both males and females.

(A-D) Gross gonadal morphology of adult male (A,C) and female (B,D) fish of both genotypes: gnrh3^+/+ (A,B) and gnrh3^-/- (C,D). (E-H) Gonadal histology with hematoxylin and eosin staining, demonstrating no differences between gnrh3^+/+ and gnrh3^-/- gametogenesis for both males and females. Male gnrh3^-/- testes contain spermatozoa in the lumens of testicular cysts (G), similar to gnrh3^+/+ testes (E). Female gnrh3^-/- ovaries contain all stages of vitellogenesis (H), including late-vitellogenic oocytes, which is similar to gnrh3^+/+ ovaries (F). White asterisks = mature spermatozoa in lumens of spermatocysts. Black stars = mature, late vitellogenic oocytes. Scale bars = 125 µm (testes) and 250 µm (ovaries).

Acknowledgments

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