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Fig. 3
A Baseline Macrophage Deficit Is Associated with an Accelerated Depletion of the Granuloma Macrophage Supply
(A and B) Absolute numbers (A) or normalized numbers (B) of macrophages in mpeg1:Gal4FF; UAS:E1bKaede larvae infected with ~240 M. marinum or mock injected. n = 5-9 larvae per group. Error bars indicate SEM.
(C) Number of macrophages in the CHT of 4-dpf mpeg1:YFP larvae 40 hr after injection of 1:10 dilution of lipo-PBS or graded doses of lipo-clodronate.
(D and E) Bacterial kinetics of cording (D) and burdens (E) in larvae infected with tdTomato-expressing M. marinum via the caudal vein at 2 dpf.
Statistical significance was determined by two-tailed unpaired Student’s t test (B) or one-way ANOVA with Sidak’s post-test (C and E). £, p = 0.02; €, p = 3 × 10-8; $, p = 0.004; and ¥, p = 0.03. (C-E) Data are representative of three independent experiments. See also Figure S4.