ZFIN Image: George et al., 2015, Fig. 7

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Figure Caption

Fig. 7

Re-expression of nkx2.5 prior to heart tube elongation results in healthy nkx2.5^-/- adults. (A) Kaplan-Meier survival curve depicts the pattern of larval death following phenotypic assessment of non-transgenic nkx2.5^-/- embryos in addition to non-transgenic and Tg(hsp70l:nkx2.5-EGFP) wild-type embryos. All animals were exposed to heat shock at 21 somites, sorted according to EGFP fluorescence, and subsequently screened morphologically at 52 hpf. While non-transgenic nkx2.5^-/- embryos perish within the first 3 weeks, 65% and 44% survival is observed in the non-transgenic and transgenic wild-type clutches, respectively. (B) Genotyping results for the non-transgenic phenotypically wild-type cohort and transgenic phenotypically wild-type cohort reveal nkx2.5^-/- embryos in the transgenic rescued group only. Percentages represent the proportion of embryos with a particular genotype in each phenotypic cohort; the number of embryos in each group is noted in parentheses.

Figure Data

Phenotype details

Acknowledgments

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Reprinted from Developmental Biology, 400(1), George, V., Colombo, S., Targoff, K.L., An early requirement for nkx2.5 Ensures first and Second heart field ventricular identity and cardiac function into adulthood, 10-22, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.