ZFIN Image: McCarthy et al., 2016, Fig. 8

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Fig. 8

Postchordal neurocranial development requires Fgf signaling during gastrulation. (A-D) Wholemount zebrafish neurocrania, anterior is to the left. (A and B) Wildtype neurocrania treated with DMSO or SU5402 develop normally. (C) DMSO-treated fgf8a mutants are also unaffected, however, (D) those treated with SU5402 from 6 to 10 hpf develop severe postchordal neurocranial loss. (E) Quantification of postchordal neurocranial defects including none (see A), ABC loss, or complete postchordal neurocranial loss (p. nc. loss) in DMSO and SU5402 treated wildtype (fgf8a+/+), heterozygous (fgf8a+/-) and mutant (fgf8a-/-) embryos. (F-H) DMSO-treated wildtype and fgf8a mutants and SU5402-treated wildtype express has2 appropriately; however, (I) SU5402-treated fgf8a mutants display a loss of expression of has2 in postchordal neurocranial precursors (arrowhead denotes most anterior expression). abc- anterior basicapsular commissure, lc-lateral commissure, n-notochord, oc-occipital arch, pc-parachordals, pbc-posterior basicapsular commissure. scale bar=20 µm in A and scale 10 µm in F.

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Acknowledgments

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Reprinted from Developmental Biology, 415(2), McCarthy, N., Sidik, A., Bertrand, J.Y., Eberhart, J.K., An Fgf-Shh signaling hierarchy regulates early specification of the zebrafish skull, 261-77, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.