ZFIN Image: Raman et al., 2016, Fig. S5

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Figure Caption

Fig. S5

MyosinII activity (pNMII levels) is higher in aPKC deficient embryos and is required for elongation of ridges in wild-type as well as in apkc morphants. pNMII staining (a) in wild-type and apkc morphant embryos at 20, 24 and 30hpf and in has/apkc mutants at 36hpf. Box-whisker plot for colocalisation coefficients (b) of F-actin and pNMII by Pearson’s methods (PC) and Manders’ overlap coefficient of pNMII with F-actin (M1) and F-actin with pNMII (M2) at 36 hpf in wild-type and has/apkc mutant embryos. Wild-type and apkc morphants (apkc MO) treated with 10µM Blebbistatin for 1.5h, fixed at 21hpf (c) and at 30hpf (d) followed by phalloidin staining to visualise F-actin. Probability values “P” are for the student’s t-test. Asterisk in (b) indicate significant difference at p<0.001. Scale bars correspond to 10 µm. Abbreviation: pNMII- phospho Non muscle-MyosinII.

Acknowledgments

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