ZFIN Image: Raman et al., 2016, Fig. S2

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Figure Caption

Fig. S2

Lgl co-localises with F-actin at microridges and exhibits temporal increase in its levels in aPKC deficient embryos. Lgl1 deficiency or Lgl2 overexpression does not perturb the overall polarity in the peridermal cells. Immuno-colocalisation using anti Lgl2 antibody and phalloidin (a) in wild-type and aPKC deficient embryos at 24hpf. Lgl2 staining in wild-type sibling and in has/apkc mutants at 32 and 48hpf (b). Confocal images along with orthogonal (X-Z) sections of ZO1 and E-cadherin stainings in wild-type embryos and lgl1 morphants at 48hpf (c). Immuno-localisation using anti aPKC and anti E-cadherin antibodies in clones expressing cmv:egfp-xlgl2 construct (d) at 30hpf. Scale bar = 10µm.

Acknowledgments

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