ZFIN Image: Gauron et al., 2016, Fig. 1

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Figure Caption

Fig. 1

Dynamics of the H₂O₂ levels during development. HyPer imaging in developing zebrafish embryos. The H₂O₂ levels are inferred from the YFP₅₀₀/YFP₄₂₀ excitation ratio of HyPer. (A) Blastula, (B-H) gastrulation. (E-H) During gastrulation, H₂O₂ is detected at the opposite side of the shield (E-H). (E, G) Bright-field images corresponding to F and H, respectively. (I) Early somitogenesis, (J) 20 somites (K) 30 hpf, (L) 48 hpf, (M) 72 hpf. Representative images are shown as lateral views, except for those in G-H (animal view). Pseudo-colour calibration bars: HyPer ratio (YFP₅₀₀/YFP₄₂₀). The bar common for A-H and the bar common for I-M allow the H₂O₂ levels in each group to be compared. Scale bars, 100 µm (A-J) and 200 µm (K-M). ot: optic tectum.

Acknowledgments

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Reprinted from Developmental Biology, 414(2), Gauron, C., Meda, F., Dupont, E., Albadri, S., Quenech'Du, N., Ipendey, E., Volovitch, M., Del Bene, F., Joliot, A., Rampon, C., Vriz, S., Hydrogen peroxide (H2O2) controls axon pathfinding during zebrafish development, 133-41, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.