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Fig. 6
Characterization of klf2a-/- mutants.
(a) anti-fibronectin staining (green) is reduced in the AVC of trpp2-/- mutants (12/12 trpp2+/+, 9/19 trpp2-/-). (b,c) A range of valvular defects were observed in klf2a-/- mutant embryos at 96 hpf (n=25 klf2a+/+, n=48 klf2a-/-), similar to those observed in klf2aMO-injected embryos (e; n=20 control, n=17 klf2aMO). (d) Photoconversion of AVC EdCs at 48 hpf shows the organization of cells within the cardiac jelly to be affected by loss of klf2a (d,e; n=21 klf2+/+, n=32 klf2a-/-) at 72 hpf. Scale bars, 10 µm.
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Full text @ Nat. Commun.