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Fig. 1
The larval zebrafish atrial wall undergoes permanent myocardial webbing. (A) Design of the priZm cassette and example spectrum of possible color combinations. (B-D) Surface myocardium of 7dpf, 14dpf and 42dpf amhc:CreER; priZm hearts, respectively, after recombination with 4-HT at 3dpf. V, ventricle; A, atrium. White arrows indicate gaps in myocardium. (E,F) 3D reconstructions of hearts from amhc:CreER; priZm animals treated with 4-HT at 3dpf and collected at 7dpf and 14dpf, respectively. Examples of individual cardiomyocytes in the atrium (A) are outlined in white. Individual cardiomyocytes in the ventricle (V) are not distinguishable. (G) Percentage of total atrial surface area not covered by myocardium at 7dpf, 14dpf and 42dpf (means±s.e.m.; n=7 hearts). Means are significantly heterogeneous (one-way ANOVA, P<0.001) and asterisks indicate means that are significantly different (Tukey-Kramer test, P<0.05). Scale bars: 50µm in B,C,E,F and 100µm in D.