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Fig. S4
MZgrk2 is fully rescued by mRNA encoding Grk2K220R and Grk2 domain swapping assay
A. In situ hybridisation of ptch2 and nkx2.2a in 24hpf wildtype (left column) and MZgrk2 embryos expressing Grk2K220RGFP (right column) (n = 30 for each sample). Scale bar, 200 µm.
B. Prox1a (green) and Eng (red) expression in the myotome of 30hpf MZgrk2 embryos injected with mRNA encoding Grk2ΔRGSGFP, Grk2ΔkinaseGFP, Grk2ΔPHGFP, Grk2ΔCmarginGFP and Grk2ΔNmarginGFP. Merge images show nuclei labelled with DAPI (blue) (n = 20). Scale bar, 50 µm.
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