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Fig. 5
Suppression of the immune response inhibits motor neuron regeneration. (A) Time line for the experiments. (B-F) Incubation with dexamethasone does not lead to visible changes in unlesioned larvae (B,C), but strongly reduces the immune reaction at the lesion site (D,E) as indicated by reduced 4C4 (arrowheads indicate 4C4+ cells) and L-plastin immunoreactivity. Quantification of immunoreactivity is shown in F (Student′s t-test, ***P<0.001). (G-I) Dexamethasone treatment reduces the number EdU-labelled Hb9:GFP+ motor neurons (arrowheads). Higher magnifications of double-labelled neurons indicated by asterisks in G,H are shown in single optical sections in G′-H′′′. (I) Quantification of the reduction in newly generated motor neurons (t-test; **P=0.0085; unlesioned, n=16; dexamethasone, n=13). Lateral views are shown; rostral is left, dorsal is up. The lesion site is indicated by a dashed line. Values are means±s.e.m. Scale bars: 100µm in E for B-E; 100µm in H for G,H; 50µm in H′′′ for G′-H′′′.