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Fig. 5

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ZDB-IMAGE-160519-21
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Figures for Huysseune et al., 2014
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Fig. 5

Figure 5. Enhanced Wnt signaling through LiCl treatment. Dentition of control zebrafish at start of treatment at 45 hpf (A), and treated with 300 mM LiCl for 1 h (B, fixed at 60 hpf and C, fixed at 112 hpf). In (B,C) only tooth 4V1 is present. Dentition of control zebrafish at start of treatment at 72 hpf (D), and treated with 300 mM LiCl for 1 h (E, fixed at 92 hpf), or treated with 300 mM LiCl for 10 min (F, fixed at 112 hpf). (E) All three first-generation teeth (3V1, 4V1, and 5V1) have formed and replacement tooth 4V2 is in morphogenesis stage (note that the section is slightly oblique and different sets of teeth are visible on either body side); (F) several tooth buds appear to form from a single enamel organ (black arrowhead, inset). Color codes for arrowheads as in Figure 3. Scale bars = 50 µm for A-F and 20 µm for inset.

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