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Fig. 7
Expression of TRPM7 dominant-negative (TRPM7-DN) suppresses differentiation by causing cell death. (A) Representative traces show the current-voltage relationship in HEK-293 cells with expression of wild-type TRPM7 alone or with coexpression of wild-type TRPM7 and the TRPM7-E1047K pore mutant (TRPM7-DN). (B) Co-expression of TRPM7-DN with wild-type TRPM7 in HEK-293 cells suppressed TRPM7 current densities at 120 mV and +100 mV. (C) Representative phase-contrast images of SH-SY5Y cells treated with retinoic acid (RA), at 4 and 6 days after plating. Shown are non-transduced (NT) cells, cells transduced with LacZ for 2 and 4 days, and cells transduced with TRPM7-DN for 2 and 4 days. Scale bar=200 μm. (D) Cell viability of non-transduced (NT), LacZ expressing, and TRPM7-DN expressing SH-SY5Y cells treated with RA 6 days after plating and 3 days post-transduction. Viability of TRPM7-DN transduced and NT cells was significantly different (Student′s t-test, p=0.006). Viability was expressed as percent of non-transduced (% NT) cells.
Reprinted from Developmental Biology, 386(2), Decker, A.R., McNeill, M.S., Lambert, A.M., Overton, J.D., Chen, Y.C., Lorca, R.A., Johnson, N.A., Brockerhoff, S.E., Mohapatra, D.P., Macarthur, H., Panula, P., Masino, M.A., Runnels, L.W., and Cornell, R.A., Abnormal differentiation of dopaminergic neurons in zebrafish trpm7 mutant larvae impairs development of the motor pattern, 428-39, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.