ZFIN Image: Decker et al., 2014, Fig. 1

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Figure Caption

Fig. 1

The TRPM7-like current is dramatically reduced in trpm7^b508 mutants. Representative whole-cell current recordings of melanophores isolated at 32 hpf, in (A) control or (B) trpm7 mutant embryos, in response to voltage ramps from -100 mV to +100 mV for 500 ms. (A) Control melanocytes exhibited outwardly rectifying currents that are potentiated by the extracellular application of 10 mM NH₄Cl, and inhibited by the application of 10 mM MgCl₂, properties that are characteristic of TRPM7-mediated currents. (B) The mutant melanophores did not exhibit any TRPM7-like outwardly rectifying currents. The slight outward rectification of currents in mutant melanophores indicates that the cells were alive. Control, n=7 cells; mutant, n=3 cells.

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Acknowledgments

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Reprinted from Developmental Biology, 386(2), Decker, A.R., McNeill, M.S., Lambert, A.M., Overton, J.D., Chen, Y.C., Lorca, R.A., Johnson, N.A., Brockerhoff, S.E., Mohapatra, D.P., Macarthur, H., Panula, P., Masino, M.A., Runnels, L.W., and Cornell, R.A., Abnormal differentiation of dopaminergic neurons in zebrafish trpm7 mutant larvae impairs development of the motor pattern, 428-39, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.