ZFIN Image: Lepanto et al., 2016, Fig. 9

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Figure Caption

Fig. 9

Embryos with impaired cilia have smaller eyes and a reduced RGC layer. a Confocal images of 48 hpf and 60 hpf retinas from embryos injected with control morpholino or elipsa-SP/ift88-SP MOs. RGCs were labeled with zn8 antibody. b and d Comparison of retinal volumes in 48 hpf (b) and 60 hpf (d) embryos. c and e Comparison of RGC layer volume (based on zn8 stain) in 48 hpf (c) and 60 hpf (e) embryos. The numbers in brackets represent the number of embryos quantified in each case (we quantified one eye per embryo). (***) p < 0.001. Comparisons were made using rank transformation and Student?s t test. nr: neural retina; le: lens; on: optic nerve. Scale bars: A, 50 �m

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