ZFIN Image: Majumdar et al., 2000, Fig. 1

Image

Figure Caption

Fig. 1

Glomerular development is altered in flh. Transverse histological sections (A, B). In wildtype zebrafish embryos at 2 days postfertilization (dpf) (A) a midline glomerulus (gl) is seen underneath the notochord (n) and dorsal aorta (da). A midline glomerulus is not seen in flh (B) at 2 dpf. Instead, clusters of cells are found in ectopic locations in flh (arrows in B). Whole-mount in situ hybridization with the wt1 probe (C-F). wt1 is expressed in nephric primordia in wildtype embryos at 20 h postfertilization (hpf) (C) and later at 48 hpf (E) in differentiated podocytes after pronephric primordia fuse in the midline. wt1 is expressed in flh nephric primordia at 20 hpf (D), but wt1-expressing cells remain in ectopic lateral positions at 48 hpf (F). Transverse sections of wt1 hybridized 36-hpf embryos (G, H). wt1-expressing cells are found at the midline in wildtype siblings (G) and in laterally positioned cells in flh (H). Some brown pigment granules are seen in these sections. Scale bars: (C, D) 200 µm; (E, F) 250 µm; (G, H) 50 µm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 222(1), Majumdar, A. and Drummond, I.A., The zebrafish floating head mutant demonstrates podocytes play an important role in directing glomerular differentiation, 147-157, Copyright (2000) with permission from Elsevier. Full text @ Dev. Biol.