ZFIN Image: Berg et al., 2016, Fig. 6

Image

Figure Caption

Fig. 6

Macrophage Lysosomal Storage Disrupts Endocytic Recycling

(A) Confocal images of red fluorescent macrophages following injection of green fluorescent dextran in E64d-treated and DMSO control larvae (3 dpf) at 5 and 30 hr post-injection. Yellow and white arrowheads denote macrophages with and without dextran, respectively. Scale bar, 50 µm.

(B) Quantification of the percentage of macrophages that are positive for dextran in E64d-treated and DMSO control larvae (3 dpf) at 5 and 30 hr post-injection.

(C) Confocal images of red fluorescent macrophages following injection of green fluorescent dextran in gba morphants and control larvae (3 dpf) at 5 and 30 hr post-injection. Yellow and white arrowheads denote macrophages with and without dextran, respectively. Scale bar, 50 µm.

(D) Quantification of the percentage of macrophages that are positive for dextran in gba morphants and control larvae (3 dpf) at 5 and 30 hr post-injection.

See also Figure S5.

Figure Data

Phenotype details

Acknowledgments

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Reprinted from Cell, 165, Berg, R.D., Levitte, S., O'Sullivan, M.P., O'Leary, S.M., Cambier, C.J., Cameron, J., Takaki, K.K., Moens, C.B., Tobin, D.M., Keane, J., Ramakrishnan, L., Lysosomal Disorders Drive Susceptibility to Tuberculosis by Compromising Macrophage Migration, 139-152, Copyright (2016) with permission from Elsevier. Full text @ Cell