Fig. 3
In situ hybridization of dio2 and dio3b on consecutive transverse cryosections of the eye of uninjected control zebrafish at 3 dpf. (A) dio2 AS: dio2 is expressed throughout all layers of the retina except IPL and most strongly in the OPL and ONL. (B) dio2 S: negative control. (C) dio3b AS: dio3b is also expressed in all retinal layers except IPL and most strongly in OPL and INL. (D) dio3b S: negative control. The different layers of the retina are indicated as GCL: ganglion cell layer; IPL: inner plexiform layer; INL: inner nuclear layer; OPL: outer plexiform layer; ONL: outer nuclear layer. AS: antisense probe; S: sense probe.
Reprinted from Molecular and Cellular Endocrinology, 424, Houbrechts, A.M., Vergauwen, L., Bagci, E., Van Houcke, J., Heijlen, M., Kulemeka, B., Hyde, D.R., Knapen, D., Darras, V.M., Deiodinase knockdown affects zebrafish eye development at the level of gene expression, morphology and function, 81-93, Copyright (2016) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.