ZFIN Image: Marelli et al., 2016, Fig. 9

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Figure Caption

Fig. 9

Analysis of the sensitivity of the TH negative feedback mechanism in thrb_MOs. Panel A) Generation of hypothyroid embryos (low T4 and high tshba) from mism_ctrls and thrb_MOs after treatment with 0.8% PTU. IHC of T4 production and WISH/Fast Blue of tshba-positive cells followed by confocal acquisition of embryos at 120 hpf (in ventral view), before and after PTU treatment. Panels B and C) ELISA quantification of T4 content and qRT-PCR of tshba in mism_ctrls and thrb_MOs before and after PTU. ***p < 0.001 vs untreated embryos. Panel D) WISH of tshba expression of the hypo_ctrls and hypo_thrb treated with increasing doses of L-T4 (10-120 nM). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

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Reprinted from Molecular and Cellular Endocrinology, 424, Marelli, F., Carra, S., Agostini, M., Cotelli, F., Peeters, R., Chatterjee, K., Persani, L., Patterns of thyroid hormone receptor expression in zebrafish and generation of a novel model of resistance to thyroid hormone action, 102-17, Copyright (2016) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.