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Fig. S2
Quantification of macrophage behavior in response to Cryptococcus during infection using mpeg macrophage marker. (a) Maximum intensity z-projection of example image data from high content imaging of Tg(mpeg1:mCherryCAAX)sh378 zebrafish, with mCherry labeled macrophages (magenta), infected with 208 cells of C. neoformans strain H99GFP (green), at 2 hours post infection. (b,c,d). Areas boxed in (a) enlarged and reconstructed in three-dimensions. 42 Arrowheads indicate intracellular cryptococci. Image pairs represent different views of same volume with arrows indicating z-axis direction. Image grid is 20µm.
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