ZFIN Image: Chang et al., 2016, Fig. 4

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Figure Caption

Fig. 4

Cyp26a1 expression at the Hb-Sc transition is under the regulation of Cdx4. In wild-types, cyp26a1 is expressed in r7/8, and in the adjacent somites 1-3 (A arrowhead). In Cdx4-deficient embryos (B), cyp26a1 expression is expanded into the spinal cord domain and terminates at the level of somite 5 (B arrowhead). Overexpression of cdx4 using Tg(hsp70:cdx4) line (C) leads to abrogation of cyp26a1 expression (D arrowheads). cyp26b1 expression (E) in r3-4 and cyp26c1 expression (G) in r2,r4,r6 remain unaffected in Cdx4-deficient embryos (F, H). (A-H) Embryos are co-stained with krx20 (r3,r5), myoD (somites) and flat-mounted at the 10 somite stage. (A, B) n=30/30 (C, D) n=15/15, (E, H) n=30/30, (I, J) n=10/10 per condition.

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Reprinted from Developmental Biology, 410(2), Chang, J., Skromne, I., Ho, R.K., CDX4 and retinoic acid interact to position the hindbrain-spinal cord transition, 178-89, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.