Fig. 1
grhl1 mRNA expression in embryonic zebrafish. (A) Phylogenetic analysis indicates that GRHL1 is evolutionarily nearer to GRHL2 than to GRHL3. (B) Grhl1 expression persists from 6 hpf onward in zebrafish. (C) Reverse-transcription PCR indicates considerable knockdown efficiency in the MO group. Primers were designed to work in intron 3 and exon 3. (D) WISH indicates a ubiquity of grhl1 in embryos at 12 and 24 hpf. The signal was detected in the ear region, al, anterior lateral line primordium; ll, lateral line neuromast; ol, olfactory placode; ov, otic vesicle; pd, pronephric duct; pf, pectoral fin; pl, posterior lateral line primordium.