ZFIN Image: Lisse et al., 2016, Fig. 2

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Figure Caption

Fig. 2

Transgenic NF-κB:EGFP zebrafish reveal peripheral NF-κB activation after H₂O₂ treatment Spatial patterns of increased NF-κB activation after H₂O₂ treatment of zebrafish larvae.

(a) 3-dpf Tg(NF-κB:EGFP) reporter zebrafish larvae at the start of the experiment. (b) Tg(NF-κB:EGFP) zebrafish were imaged after 2hr post 0.01% H₂O₂ treatment. Both whole larvae (upper) and tail fins (below) were imaged, whereby GFP⁺ cells were partially localized to the periphery (arrows). (c) Quantitative analysis using relative mean fluorescence of the z-stack projected images using ImageJ (n = 2, 10 fish). Observation of increased number of GFP-labeled cells and overall fluorescence intensity in the whole larvae. (d) Higher-resolution analyses of the tail fin revealed a peripheral tissue spatial pattern of increase NF-κB activated cells after H₂O₂ treatment. n = 3 independent experiments per condition.

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Acknowledgments

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