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Fig. S3
Changes in levels of total or phosphorylated SFKs, and phospho-tyrosine upon PrP-1 knockdown. A. SFK levels in 6 hpf embryos (WB) after injection with increasing PrP-1 morpholino doses. B. Changes in relative p-Tyr527 levels in PrP-1 morphants, as measured by densitometric analysis of WB bands. WB for total or p-Tyr527 SFKs was performed with 6 hpf embryo extracts. Values for phospho Y527 SFKs were normalized to those of total SFKs. Average values ± SEM of three independent experiments are depicted. C. Immunofluorescence of total phosphorylated tyrosine in deep and EVL cells in 6 hpf embryos. Scale bar = 10 µM.