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Fig. 7
Morphological analysis and quantifications of human mRNAs injected embryos.
(A) Toluidine blue-stained transverse sections and electron micrographs at 4 dpf of DNM2_mutCNM and DNM2_mutCMT -injected embryos in comparison to control (DNM2_ctrl). In Toluidine blue-stained sections both CNM and CMT injected embryos muscle tissue has more disordered morphology than control embryos, with fibres of variable size, frequent lobular appearance and more space surrounding fibres. Scale bar = 10 µm. Electron micrographs show greater vesicular decoration in spaces surrounding fibres (asterisks) and loose morphology. Scale bar = 0.5 µm. (B) Quantitation of fibre diameter indicates that the distribution of fibre diameter is shifted towards larger diameter in CNM- and CMT-injected embryos compared to control (in DNM2_mutCNM and DNM2_mutCMT 30.2% and 17.3% respectively of fibre diameters were 10.1 µm or greater, compared to 13.3%in control p < 0.0001). (C) Quantitation of central nuclei per fibre shows significantly more central nuclei in both CNM- and CMT-injected embryos than control. Morphological analysis were performed on n = 6 embryos for DNM2_mutCNM, DNM2_mutCMT and DNM2_ctrl each, chosen randomly from 4 different injections.