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Fig. S5
Cell death does not contribute to the reduction of liver size upon fhl1b depletion.
(A-B) TUNEL labeling (red) combined with anti-Prox1 immunostaining (grey) revealed that no TUNEL-positive liver cells were observed both in fhl1b morphants and control embryos at 48 hpf. (C-C′) As a control, Tg(fabp10a:CFP-NTR)gt1 embryos were used. Treating metronidazole (MTZ) caused apoptosis in a large number of hepatocytes. A-C′, confocal projection images, ventral views, anterior to the top. Scale bar, 20 µm.
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