ZFIN Image: Govindan et al., 2016, Fig. S2

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Figure Caption

Fig. S2

Heat shock induces upregulation of sema3d mRNA and Sema3d protein in Tg(hsp70:sema3d-gfp).

(A) Whole mount in situ hybridization shows increased expression of sema3d mRNA in heat shock treated fins compared to the untreated fins. Black line indicates amputation plane. (B) Immuno-staining analysis of longitudinal fin sections reveal increased expression of GFP (green) and Sema3d (Red) in heat shock treated fins compared to the untreated fins. DAPI (blue) is used as the counter stain and stains the nuclei. Arrows indicate basal layer of epithelium and arrow head marks the bone. e, epidermis; m, mesenchyme. Scale bar represents 100µm in both panels.

Acknowledgments

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