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Fig. S4
Early neural plate patterning appears normal in six3a;six3b double mutants. (A-D) Wild-type embryos (A,C) and embryos from crosses between six3a;six3b double heterozygous parents (n>100 for each labelling) (B,D) were labelled by in situ hybridization for eye field marker rx3 (A,B) and neural plate marker fezf2 (C,D) at early segmentation. All embryos in clutches from six3a;six3b double heterozygous parent appeared similar and comparable to wild type. (C,D) Arrows and arrowheads point at presumptive telencephalon and ventral diencephalon, respectively.
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