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Fig. 4
smFISH provides quantitative subcellular information on gene expression. Detected transcripts (magenta) and transcription foci (white) for eif4g2a (A), tbx16 (B) and akap12b (C) at sphere stage. Green, cell outlines; blue, nuclear outlines. Scale bars: 10µm. Images are maximum projections of 17 z-slices spaced by 0.3µm. (D) Single transcript quantification. Values are averages from sections of three different embryos. Error bars represent s.e.m. (E) smFISH for akap12b (left) with corresponding membrane staining for Phalloidin (right) at dome stage. Images are single z-slices. Scale bars: 10 µm.
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