Fig. S6
Normal dorsoventral patterning in embryos with lbx1b overexpression.
(A) Whole-mount in situ hybridization (WISH) for dorsal organizer gene chd and ventral gene vox in shield-stage embryos injected with buffer as control (ctrl) or lbx1b mRNA (lbx1b). Photos are taken from the animal pole side of embryos with dorsal to the left. (B) Quantitative analysis of the WISH signals shown in A. For quantification of the dorsoventral position, the angle θ [degree] shown in the panel A as measured. No significant change was observed for both chd (p = 0.261) and vox (p = 0.071) expression pattern. The numbers of control and lbx1b zebrafish embryos were 22 and 23 for chd, 23 and 23 for vox, respectively.