ZFIN Image: Ye et al., 2016, Fig. S2

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Figure Caption

Fig. S2

Insulin signaling blockade via dnIRS2-GFP mis-expression and drug treatments. (A) Peptide sequence of dnIRS2-GFP transgene showing pleckstrin homology (PH), phosphotyrosine binding (PTB) and GFP domains. (B) Control and dnIRS2-GFP mRNAinjected embryos at 24 hours post fertilization (hpf). (C,D) In situ hybridization showing pdx1 expression in control (C) and dnIRS2-GFP mRNA-injected (D) embryos. pdx1 expression is expanded with insulin signaling blockade. (E,F) Confocal projections of 30 hpf Tg(ins:CFP-NTR) pancreatic islets treated with DMSO as a vehicle control (E) or 1µM wortmannin (F). (G) Quantification of β cells in DMSO-treated control (n=10) and 100 µM U0126-treated (n=9) islets at 32 hpf. No change in β cell number was observed with U0126-mediated MAPK inhibition. Student’s t-test was used to determine significance in G.

Acknowledgments

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Reprinted from Developmental Biology, 409(2), Ye, L., Robertson, M.A., Mastracci, T.L., Anderson, R.M., An insulin signaling feedback loop regulates pancreas progenitor cell differentiation during islet development and regeneration, 354-69, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.