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Fig. S15
Transplantation of ECBs influences host β cell regeneration in the principal islet. (A-C) Confocal projections of host-derived regenerating principal islets within chimeras at 2 days post ablation. Chimera composition was: (A-A′′) Sham donor control without transplantation (B-B′′); control donor Tg(sox17:GFP) ECBs; and (C-C′′) dnIRS2-GFP mRNAinjected donor Tg(sox17:GFP) ECBs. Chimeras were immunostained for Insulin (red), Glucagon (white), and DNA (blue). Regeneration of β cells was increased in the principal islet of the host when insulin signaling was blocked by dnIRS2-GFP in the transplanted ECBs.
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Reprinted from Developmental Biology, 409(2), Ye, L., Robertson, M.A., Mastracci, T.L., Anderson, R.M., An insulin signaling feedback loop regulates pancreas progenitor cell differentiation during islet development and regeneration, 354-69, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.