ZFIN Image: Ye et al., 2016, Fig. S12

Image

Figure Caption

Fig. S12

Insulin knockdown has no effect on δ cell number or total endocrine cell number. (A,B) Confocal projections of 3 dpf control (A) and insaMO-injected (B) islets immunostained for Insulin (green), Somatostatin (red), and DNA (blue). (C) Quantification of somatostatin-positive cells in control (n=5) and insMO-injected (n=6) embryos at 3 dpf. No significant change was evident in δ cell mass. (D) Quantification of endocrine cells in control and insaMO-injected embryos from 1 dpf to 4 dpf. n≥7 for all time points. (E-L) Confocal projections of control (E-H) and insaMO (I-L) islets in doubly transgenic Tg(neurod:GFP); Tg(ins:dsRed)embryos. Student’s t-test was used in C and two way ANOVA was used in D to determine significance.

Acknowledgments

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Reprinted from Developmental Biology, 409(2), Ye, L., Robertson, M.A., Mastracci, T.L., Anderson, R.M., An insulin signaling feedback loop regulates pancreas progenitor cell differentiation during islet development and regeneration, 354-69, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.