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Fig. 2
Tg(FLEx-lima1a:GFP) recapitulates endogenous lima1a expression. (A) Schematic representation of SAGFLEx integrated into intron 2 of the lima1a locus in Tg(FLEx-lima1a:GFP). Native GFP fluorescence (B–F) and gfp transcription (G–K) in Tg(FLEx-lima1a:GFP) compared to lima1a expression in wildtype embryos (L–P). Lateral views at 12 somite stage (15 hpf), 20 somite stage (19 hpf), 24, 30, and 48 hpf. Dorsal views of single and double in situ hybridizations for lima1a and gfp at 30 hpf (Q–S).
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