ZFIN Image: Duncan et al., 2016, Fig. 6

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Figure Caption

Fig. 6

Wnt/β-catenin signaling activation blocks expansion of the radial glial population. (A) Induction of Wnt8a at 5dpf leads to a decrease in the number of GS⁺ radial glia at 6dpf. (B) Addition of 4µM BIO, a Gsk3β inhibitor, daily from 6-9dpf leads to a decrease in GS⁺ radial glia. (C) Representative images of labeling for GS in control and BIO-treated larvae. Images are single optical sections from ventral views of whole-mount brains. Scale bar: 10µm. (D) Following recombination from 5-6dpf, incubation in BIO causes a significant decrease in the number of labeled progeny at 9dpf. (E) Incubation in BIO causes a significant increase in the percentage of GS⁺ cells within the labeled lineage. Error bars indicate s.e.m.; n=40 optical sections from four brains for each experiment.

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