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Fig. 3
Wnt/β-catenin signaling is only required for the differentiation of a specific subset of ventricular neurons. (A,B) Following recombination of 3her4.1:ERT2-Cre-ERT+ progeny from 5-6dpf, expression of Dkk1 from 6-9dpf does not affect the average number of labeled cells (A) or the percentage of GS+ cells (B) in the lineage. (C) The differentiation of HuC/D+ neurons from labeled radial glia is not inhibited by Dkk1 expression. (D) Representative images of labeling for lineage and GS in control and Dkk1-expressing larvae. (E) In lef1 mutants there is no change in the percentage of GS+ cells after conversion from 5-6dpf and analysis at 9dpf. (F,G) lef1-dependent ventricular neurons fail to arise from the radial glial lineage, whereas other non-ventricular neurons are not significantly affected. Yellow boxes indicate areas shown to the right. Images are single optical sections from ventral views of whole-mount brains. Error bars indicate s.e.m.; n=50 confocal slices from five brains for each experiment. Scale bars: 10µm.