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Fig. S7
F-actin cortex at the vegetal pole is similar in wild-type and hec mutant embryos. At 20 mpf, both wild-type (A) and mutant (B) eggs show F-actin rich folds and villi-like structures, which may correspond to previously described microplicae [45]. Number of embryos tested: 18 wt (from a pool of four females) and 24 mutants (from two different mutant females). A fraction (21%, n = 24) of hec mutant embryos show radial F-actin enrichments (C), correlating with aster-like microtubule structures in these embryos (Figure S5). Magnification bar in (C) corresponds to 40 µm in all panels.
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