ZFIN Image: Yokota et al., 2015, Fig. 2 S2

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Figure Caption

Fig. 2 S2

Vegfr3 is not involved in Ca2+ oscillations in tip cells budding from the dorsal aorta (DA).

(A) 3D-rendered time-sequential images of Tg(fli1:Gal4FF);(UAS:GCaMP7a);(fli1:H2B-mC) embryos injected with vegfr3 MO at 25 ss. A green arrowhead indicates a budding tip cell. (B) The fluorescence changes in GCaMP7a (ΔF/F₀) of individual ECs from A indicated by arrowheads at the left panel are shown as a graph. (C) Quantification of Ca²⁺ oscillatory activity of vegfr3 morphants at 24–27 ss. Graphs show Ca²⁺ oscillation frequency (left) and mean ΔF/F₀ (right) of tip cells and other ECs within the DA during tip cell budding (n ≥ 12). Scale bar, 10 µm in A. **p < 0.01, ***p < 0.001; NS, not significant.

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